medsovetМедицинский СоветMeditsinskiy sovet = Medical Council2079-701X2658-5790REMEDIUM GROUP Ltd.10.21518/2079-701X-2017-15-61-67medsovet-2066Research ArticleЗАБОЛЕВАНИЯ КИШЕЧНИКАBOWEL DISEASESРОЛЬ МИКРОБИОТЫ В ЖИЗНИ ЧЕЛОВЕКА И ПЕРСПЕКТИВЫ ПРОФИЛАКТИЧЕСКОГО ПРИМЕНЕНИЯ ПРОБИОТИКОВROLE OF MICROBIOTA IN HUMAN LIFE AND THE PROSPECTS FOR THE PREVENTIVE USE OF PROBIOTICSЗахаренкоС. М.ZakharenkoS. M.

к.м.н.

PhD in medicine

Военно-медицинская академия им. С.М. Кирова Минобороны РоссииРоссияKirov Military and Medical Academy of the Ministry of Defense of the Russian FederationRussian Federation2017041120170156167Copyright © Захаренко С.М., 20172017Захаренко С.М.Zakharenko S.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-sovet.pro/jour/article/view/2066

Концепция про- и пребиотической терапии находит как своих сторонников, так и противников. Вместе с тем не вызывает сомнений тесная связь между микробиотой различных эпитопов нашего организма и функционированием как отдельных его органов и систем, так и всего организма в целом. Накопленный объем достоверных данных о функционировании микробиоценозов организма человека позволяет рассматривать их как ключевые точки влияния на здоровье человека не только в плане лечения имеющихся заболеваний, но и в плане первичной и вторичной профилактики широкого спектра инфекционных, метаболических, иммуноопосредованных и иных заболеваний. В ближайшем будущем предстоит определить показания, найти эффективные пробиотические штаммы, определить оптимальные разовые и суточные дозы, продолжительность приема, а также критерии эффективности проводимых мероприятий.

 

The concept of pro- and prebiotic therapy finds both its followers and opponents. However, there is no doubt that there is a close connection between the microbiota of the various epitopes of our organism and the functioning of its individual organs and systems, as well as of the body as a whole. The accumulated amount of reliable data on the functioning of the microbiocenosis of the human organism makes it possible to see them as key points of influence on human health, not only in the treatment of existing diseases, but also in terms of primary and secondary prevention of a wide range of infectious, metabolic, immune-mediated and other diseases. In the near future, scientists would need to identify the indications, find effective probiotic strains, determine optimal one-time and daily doses, duration of the therapy, and criteria for effectiveness of the therapeutic and/or preventative measures.

 

микробиотапробиотикипрофилактикаmicrobiotaprobioticspreventionReferencesWhitman WB, Coleman DC, Wiebe WJ. Prokaryotes: the unseen majority. Proc. Natl. Acad. Sci. USA, 1998, 95: 6578–6583.Whitman WB, Coleman DC, Wiebe WJ. Prokaryotes: the unseen majority. Proc. Natl. Acad. Sci. USA, 1998, 95: 6578–6583.Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. PLoS Biol, 2016, 14: e1002533.Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. PLoS Biol, 2016, 14: e1002533.Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science, 1999 May 21, 284(5418): 1318-22.Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science, 1999 May 21, 284(5418): 1318-22.Rodríguez JM, Murphy K, Stanton C, Ross RP, Kober OI, Juge N, Avershina E, Rudi K, Narbad A, Jenmalm MC, Marchesi JR, Collado MC. The composition of the gut microbiota throughout life, with an emphasis on early life. Microb Ecol Health Dis, 2015 Feb 2, 26: 26050. doi: 10.3402/ mehd.v26.26050.Rodríguez JM, Murphy K, Stanton C, Ross RP, Kober OI, Juge N, Avershina E, Rudi K, Narbad A, Jenmalm MC, Marchesi JR, Collado MC. The composition of the gut microbiota throughout life, with an emphasis on early life. Microb Ecol Health Dis, 2015 Feb 2, 26: 26050. doi: 10.3402/ mehd.v26.26050.Vaidyaa YH, Patela SA, Patela RJ, Panditb RJ, Joshib CJ, Kunjadiac AP. Human milk microbiome in urban and rural populations of India. Meta Gene, 2017, 13: 13-22.Vaidyaa YH, Patela SA, Patela RJ, Panditb RJ, Joshib CJ, Kunjadiac AP. Human milk microbiome in urban and rural populations of India. Meta Gene, 2017, 13: 13-22.Lehtimäki J, Karkman A, Laatikainen T, Paalanen L, von Hertzen L, Haahtela T, Hanski I, Ruokolainen L. Patterns in the skin microbiota differ in children and teenagers between rural and urban environments. Sci Rep, 2017, 7: 45651.Lehtimäki J, Karkman A, Laatikainen T, Paalanen L, von Hertzen L, Haahtela T, Hanski I, Ruokolainen L. Patterns in the skin microbiota differ in children and teenagers between rural and urban environments. Sci Rep, 2017, 7: 45651.Привольнев В.В., Захаренко С.М., Андреева И.В. Новые данные о роли пробиотиков в восстановлении поврежденной антибиотиками микрофлоры кишечника. Лечение и профилактика, 2016, 3(19): 39-48.Привольнев В.В., Захаренко С.М., Андреева И.В. Новые данные о роли пробиотиков в восстановлении поврежденной антибиотиками микрофлоры кишечника. Лечение и профилактика, 2016, 3(19): 39-48.Moeller AH, Caro-Quintero A, Mjungu D, Georgiev AV, Lonsdorf EV, Muller MN, Pusey AE, Peeters M, Hahn BH, Ochman H. Cospeciation of gut microbiota with hominids. Science, 2016, 353: 380-382.Moeller AH, Caro-Quintero A, Mjungu D, Georgiev AV, Lonsdorf EV, Muller MN, Pusey AE, Peeters M, Hahn BH, Ochman H. Cospeciation of gut microbiota with hominids. Science, 2016, 353: 380-382.Gomez A, Petrzelkova KJ, Burns MB, Yeoman CJ, Amato KR, Vlckova K, Modry D, Todd A, Robinson CAJ, Remis MJ et al. Gut microbiome of coexisting BaAka Pygmies and Bantu reflects gradients of traditional subsistence patterns. Cell Rep, 2016, 14: 2142-2153.Gomez A, Petrzelkova KJ, Burns MB, Yeoman CJ, Amato KR, Vlckova K, Modry D, Todd A, Robinson CAJ, Remis MJ et al. Gut microbiome of coexisting BaAka Pygmies and Bantu reflects gradients of traditional subsistence patterns. Cell Rep, 2016, 14: 2142-2153.Obregon-Tito AJ, Tito RY, Metcalf J, Sankaranarayanan K, Clemente JC, Ursell LK, Xu ZZ, Van Treuren W, Knight R, Gaffney PM et al. Subsistence strategies in traditional societies distinguish gut microbiomes. Nat. Commun, 2015, 6: 6505.Obregon-Tito AJ, Tito RY, Metcalf J, Sankaranarayanan K, Clemente JC, Ursell LK, Xu ZZ, Van Treuren W, Knight R, Gaffney PM et al. Subsistence strategies in traditional societies distinguish gut microbiomes. Nat. Commun, 2015, 6: 6505.Morton ER, Lynch J, Froment A, Lafosse S, Heyer E, Przeworski M, Blekhman R, Segurel L. Variation in rural African gut microbiota is strongly correlated with colonization by Entamoeba and subsistence. PLoS Genet, 2015, 11: e1005658.Morton ER, Lynch J, Froment A, Lafosse S, Heyer E, Przeworski M, Blekhman R, Segurel L. Variation in rural African gut microbiota is strongly correlated with colonization by Entamoeba and subsistence. PLoS Genet, 2015, 11: e1005658.Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, Magris M, Hidalgo Baldassano G, RN, Anokhin AP et al. Human gut microbiome viewed across age and geography. Nature, 2012, 486: 222-227.Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, Magris M, Hidalgo Baldassano G, RN, Anokhin AP et al. Human gut microbiome viewed across age and geography. Nature, 2012, 486: 222-227.Clemente JC, Pehrsson EC, Blaser MJ, Sandhu K, Gao Z, Wang B, Magris M, Hidalgo G, Contreras M, Noya-Alarcón Ó et al. The microbiome of uncontacted Amerindians. Sci. Adv, 2015, 1: e1500183.Clemente JC, Pehrsson EC, Blaser MJ, Sandhu K, Gao Z, Wang B, Magris M, Hidalgo G, Contreras M, Noya-Alarcón Ó et al. The microbiome of uncontacted Amerindians. Sci. Adv, 2015, 1: e1500183.Martínez I, Stegen JC, Maldonado-Gómez MX, Eren AM, Siba PM, Greenhill AR, Walter J. The gut microbiota of rural Papua New Guineans: composition, diversity patterns, and ecological processes. Cell Rep, 2015, 11: 527-538.Martínez I, Stegen JC, Maldonado-Gómez MX, Eren AM, Siba PM, Greenhill AR, Walter J. The gut microbiota of rural Papua New Guineans: composition, diversity patterns, and ecological processes. Cell Rep, 2015, 11: 527-538.Tyakht AV, Kostryukova ES, Popenko AS, Belenikin MS, Pavlenko AV, Larin AK, Karpova IY, Selezneva OV, Semashko TA, Ospanova EA. Human gut micro-biota community structures in urban and rural populations in Russia. Nat. Commun, 2013, 4: 2469.Tyakht AV, Kostryukova ES, Popenko AS, Belenikin MS, Pavlenko AV, Larin AK, Karpova IY, Selezneva OV, Semashko TA, Ospanova EA. Human gut micro-biota community structures in urban and rural populations in Russia. Nat. Commun, 2013, 4: 2469.Liefert W. Food security in Russia: economic growth and rising incomes are reducing insecurity. Food Security Assess, 2004: 35-43.Liefert W. Food security in Russia: economic growth and rising incomes are reducing insecurity. Food Security Assess, 2004: 35-43.Parashar A, Udayabanu M. Gut microbiota regulates key modulators of social behavior. Eur Neuropsychopharmacol, 2016 Jan, 26(1): 78-91. doi: 10.1016/j.euroneuro.2015.11.002.Parashar A, Udayabanu M. Gut microbiota regulates key modulators of social behavior. Eur Neuropsychopharmacol, 2016 Jan, 26(1): 78-91. doi: 10.1016/j.euroneuro.2015.11.002.Gibson GR. Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition Supplements, 2004, 1(2): 25-31.Gibson GR. Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition Supplements, 2004, 1(2): 25-31.Flint HJ, Bayer EA, Rincon MT, Lamed R, White BA. Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis. Nat Rev Microbiol, 2008 Feb, 6(2): 121-31. doi: 10.1038/nrmicro1817.Flint HJ, Bayer EA, Rincon MT, Lamed R, White BA. Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis. Nat Rev Microbiol, 2008 Feb, 6(2): 121-31. doi: 10.1038/nrmicro1817.Walker AW, Duncan SH, Harmsen HJM, Holtrop G, Welling GW, Flint HJ. The species composition of the human intestinal microbiota differs between particle-associated and liquid phase communities. Environ. Microbiol., 2008, 10: 3275-3283.Walker AW, Duncan SH, Harmsen HJM, Holtrop G, Welling GW, Flint HJ. The species composition of the human intestinal microbiota differs between particle-associated and liquid phase communities. Environ. Microbiol., 2008, 10: 3275-3283.Ze X, Duncan SH, Louis P, Flint HJ. Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon. ISME J, 2012, 6: 1535-1543.Ze X, Duncan SH, Louis P, Flint HJ. Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon. ISME J, 2012, 6: 1535-1543.Ze X, Ben DY, Laverde-Gomez JA, Dassa B, Sheridan PO, Duncan SH, Louis P, Henrissat B, Juge N, Koropatkin NM et al.: Unique organization of extracellular amylases into amylosomes in the resistant starch-utilizing human colonic Firmicutes bacterium Ruminococcus bromii. mBio, 2015, 6: e01058–15.Ze X, Ben DY, Laverde-Gomez JA, Dassa B, Sheridan PO, Duncan SH, Louis P, Henrissat B, Juge N, Koropatkin NM et al.: Unique organization of extracellular amylases into amylosomes in the resistant starch-utilizing human colonic Firmicutes bacterium Ruminococcus bromii. mBio, 2015, 6: e01058–15.Ben DY, Dassa B, Borovok I, Lamed R, Koropatkin NM, Martens EC, White BA, Bernalier-Donadille A, Duncan SH, Flint HJ et al. Ruminococcal cellulosome systems from rumen to human. Environ. Microbiol., 2015, 17: 3407-3426.Ben DY, Dassa B, Borovok I, Lamed R, Koropatkin NM, Martens EC, White BA, Bernalier-Donadille A, Duncan SH, Flint HJ et al. Ruminococcal cellulosome systems from rumen to human. Environ. Microbiol., 2015, 17: 3407-3426.Brigidi P. IBS Bologna Days. 2016 June 8-10.Brigidi P. IBS Bologna Days. 2016 June 8-10.Mahowald MA et al. Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla. Proc Natl Acad Sci U S A, 2009, 106(14): 5859-5864.Mahowald MA et al. Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla. Proc Natl Acad Sci U S A, 2009, 106(14): 5859-5864.Muñoz-Tamayo R, Laroche B, Walter E, Doré J, Duncan SH, Flint HJ, Leclerc M. Kinetic modelling of lactate utilization and butyrate production by key humancolonic bacterial species. FEMS Microbiol Ecol., 2011, 76(3): 615-624.Muñoz-Tamayo R, Laroche B, Walter E, Doré J, Duncan SH, Flint HJ, Leclerc M. Kinetic modelling of lactate utilization and butyrate production by key humancolonic bacterial species. FEMS Microbiol Ecol., 2011, 76(3): 615-624.Fu J, Bonder MJ, Cenit MC, Tigchelaar EF, Maatman A, Dekens JA, Brandsma E, Marczynska J, Imhann F, Weersma RK, Franke L, Poon TW, Xavier RJ, Gevers D, Hofker MH, Wijmenga C, Zhernakova A. The Gut Microbiome Contributes to a Substantial Proportion of the Variation in Blood Lipids. Circ Res, 2015 Oct 9, 117(9): 817-24. doi: 10.1161/CIRCRESAHA.115.306807.Fu J, Bonder MJ, Cenit MC, Tigchelaar EF, Maatman A, Dekens JA, Brandsma E, Marczynska J, Imhann F, Weersma RK, Franke L, Poon TW, Xavier RJ, Gevers D, Hofker MH, Wijmenga C, Zhernakova A. The Gut Microbiome Contributes to a Substantial Proportion of the Variation in Blood Lipids. Circ Res, 2015 Oct 9, 117(9): 817-24. doi: 10.1161/CIRCRESAHA.115.306807.Drosos I, Tavridou A, Kolios G. New aspects on the metabolic role of intestinal microbiota in the development of atherosclerosis. Metabolism. 2015 Apr, 64(4): 476-81. doi: 10.1016/j.metabol.2015.01.007.Drosos I, Tavridou A, Kolios G. New aspects on the metabolic role of intestinal microbiota in the development of atherosclerosis. Metabolism. 2015 Apr, 64(4): 476-81. doi: 10.1016/j.metabol.2015.01.007.Gensollen T, Iyer SS, Kasper DL, Blumberg RS. How colonization by microbiota in early life shapes the immune system. Science, 2016, 352: 539-544.Gensollen T, Iyer SS, Kasper DL, Blumberg RS. How colonization by microbiota in early life shapes the immune system. Science, 2016, 352: 539-544.Hand TW, Vujkovic-Cvijin I, Ridaura VK, Belkaid Y. Linking the Microbiota, Chronic Disease, and the Immune System. Trends Endocrinol Metab, 2016 Dec, 27(12): 831-843. doi: 10.1016/j. tem.2016.08.003.Hand TW, Vujkovic-Cvijin I, Ridaura VK, Belkaid Y. Linking the Microbiota, Chronic Disease, and the Immune System. Trends Endocrinol Metab, 2016 Dec, 27(12): 831-843. doi: 10.1016/j. tem.2016.08.003.Corrêa-Oliveira R, Fachi JL, Vieira A, Sato FT, Vinolo M.A.R. Regulation of immune cell function by short-chain fatty acids. Clin Transl Immunology, 2016 Apr, 5(4): e73. doi: 10.1038/ cti.2016.17.Corrêa-Oliveira R, Fachi JL, Vieira A, Sato FT, Vinolo M.A.R. Regulation of immune cell function by short-chain fatty acids. Clin Transl Immunology, 2016 Apr, 5(4): e73. doi: 10.1038/ cti.2016.17.Elinav E, Strowig T, Kau AL, Henao-Mejia J, Thaiss CA, Booth CJ, Peaper DR, Bertin J, Eisenbarth SC, Gordon JI, Flavell RA. NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis. Cell, 2011 May 27, 145(5): 745-57. doi: 10.1016/j.cell.2011.04.022.Elinav E, Strowig T, Kau AL, Henao-Mejia J, Thaiss CA, Booth CJ, Peaper DR, Bertin J, Eisenbarth SC, Gordon JI, Flavell RA. NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis. Cell, 2011 May 27, 145(5): 745-57. doi: 10.1016/j.cell.2011.04.022.Frantz AL, Rogier EW, Weber CR, Shen L, Cohen DA, Fenton LA, Bruno ME, Kaetzel CS. Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol, 2012 Sep, 5(5): 501-12. doi: 10.1038/mi.2012.23.Frantz AL, Rogier EW, Weber CR, Shen L, Cohen DA, Fenton LA, Bruno ME, Kaetzel CS. Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol, 2012 Sep, 5(5): 501-12. doi: 10.1038/mi.2012.23.Sonnenberg GF1, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Tardif MR, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang KM, Artis D. Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria. Science, 2012 Jun 8, 336(6086): 1321-5. doi: 10.1126/science.1222551.Sonnenberg GF1, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Tardif MR, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang KM, Artis D. Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria. Science, 2012 Jun 8, 336(6086): 1321-5. doi: 10.1126/science.1222551.Hirata So-ichiro, Kunisawa J. Gut microbiome, metabolome, and allergic diseases. Allergology International, 2017. http://dx.doi.org/10.1016/j. alit.2017.06.008.Hirata So-ichiro, Kunisawa J. Gut microbiome, metabolome, and allergic diseases. Allergology International, 2017. http://dx.doi.org/10.1016/j. alit.2017.06.008.Pope JL, Tomkovich S, Yang Y, Jobin C (2016) Microbiota as a mediator of cancer progression and therapy. Transl Res. doi:10.1016/j. trsl.2016.07.021. http://www.translationalres. com/article/S1931-5244(16)30155-4/fulltext.Pope JL, Tomkovich S, Yang Y, Jobin C (2016) Microbiota as a mediator of cancer progression and therapy. Transl Res. doi:10.1016/j. trsl.2016.07.021. http://www.translationalres. com/article/S1931-5244(16)30155-4/fulltext.Kurilshikov A, Wijmenga C, Fu J, Zhernakova A. Host Genetics and Gut Microbiome: Challenges and Perspectives. Trends in Immunology, 2017. http://dx.doi.org/10.1016/j.it.2017.06.003.Kurilshikov A, Wijmenga C, Fu J, Zhernakova A. Host Genetics and Gut Microbiome: Challenges and Perspectives. Trends in Immunology, 2017. http://dx.doi.org/10.1016/j.it.2017.06.003.Goodrich JK, Davenport ER, Beaumont M, Jackson MA, Knight R, Ober C, Spector TD, Bell JT, Clark AG, Ley RE. Genetic Determinants of the Gut Microbiome in UK Twins. Cell Host Microbe, 2016 May 11, 19(5): 731-43. doi: 10.1016/j.chom.2016.04.017.Goodrich JK, Davenport ER, Beaumont M, Jackson MA, Knight R, Ober C, Spector TD, Bell JT, Clark AG, Ley RE. Genetic Determinants of the Gut Microbiome in UK Twins. Cell Host Microbe, 2016 May 11, 19(5): 731-43. doi: 10.1016/j.chom.2016.04.017.Ghaisas S, Maher J, Kanthasamy A. Gut microbiome in health and disease: Linking the microbiome-gut-brain axis and environmental factors in the pathogenesis of systemic and neurode-generative diseases. Pharmacol Ther, 2016, 158: 52-62. doi: 10.1016/j.pharmthera.2015.11.012.Ghaisas S, Maher J, Kanthasamy A. Gut microbiome in health and disease: Linking the microbiome-gut-brain axis and environmental factors in the pathogenesis of systemic and neurode-generative diseases. Pharmacol Ther, 2016, 158: 52-62. doi: 10.1016/j.pharmthera.2015.11.012.Faith JJ, Guruge JL, Charbonneau M, Subramanian S, Seedorf H et al. The long-term stability of the human gut microbiota. Science, 2013, 341: 1237439.Faith JJ, Guruge JL, Charbonneau M, Subramanian S, Seedorf H et al. The long-term stability of the human gut microbiota. Science, 2013, 341: 1237439.Ситкин С.И., Ткаченко Е.И., Вахитов Т.Я. Метаболический дисбиоз и его биомаркеры. Экспериментальная и клиническая гастроэнтерология, 2015, 12(124): 6-29.Ситкин С.И., Ткаченко Е.И., Вахитов Т.Я. Метаболический дисбиоз и его биомаркеры. Экспериментальная и клиническая гастроэнтерология, 2015, 12(124): 6-29.Probiotics and prebiotics. Practice Guideline. World Gastroenterology Organisation. February 2017, 35 p.Probiotics and prebiotics. Practice Guideline. World Gastroenterology Organisation. February 2017, 35 p.Khoder G, Al-Menhali AA, Al-Yassir F, Karam SM. Exp Ther Med. 2016 Jul, 12(1): 3-17.Khoder G, Al-Menhali AA, Al-Yassir F, Karam SM. Exp Ther Med. 2016 Jul, 12(1): 3-17.van den Nieuwboer M, Browne PD, Claassen E. Patient needs and research priorities in probiotics: A quantitative KOL prioritization analysis with emphasis on infants and children. Pharma Nutrition, 2016, 4(1): 19–28.van den Nieuwboer M, Browne PD, Claassen E. Patient needs and research priorities in probiotics: A quantitative KOL prioritization analysis with emphasis on infants and children. Pharma Nutrition, 2016, 4(1): 19–28.Lammers KM, Brigidi P, Vitali B, Gionchetti P, Rizzello F, Caramelli E, Matteuzzi D, Campieri M. Immunomodulatory effects of probiotic bacteria DNA: IL-1 and IL-10 response in human peripheral blood mononuclear cells. FEMS Immunol Med Microbiol, 2003 Sep 22, 38(2): 165-72.Lammers KM, Brigidi P, Vitali B, Gionchetti P, Rizzello F, Caramelli E, Matteuzzi D, Campieri M. Immunomodulatory effects of probiotic bacteria DNA: IL-1 and IL-10 response in human peripheral blood mononuclear cells. FEMS Immunol Med Microbiol, 2003 Sep 22, 38(2): 165-72.Paineau D, Carcano D, Leyer G, Darquy S, Alyanakian MA, Simoneau G, Bergmann JF, Brassart D, Bornet F, Ouwehand AC. Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunol Med Microbiol, 2008 Jun, 53(1): 107-13. doi: 10.1111/j.1574-695X.2008.00413.x.Paineau D, Carcano D, Leyer G, Darquy S, Alyanakian MA, Simoneau G, Bergmann JF, Brassart D, Bornet F, Ouwehand AC. Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunol Med Microbiol, 2008 Jun, 53(1): 107-13. doi: 10.1111/j.1574-695X.2008.00413.x.Taipale T, Pienihäkkinen K, Isolauri E, Larsen C, Brockmann E, Alanen P, Jokela J, Söderling E. Bifidobacterium animalis subsp. lactis BB-12 in reducing the risk of infections in infancy. Br J Nutr, 2011 Feb, 105(3): 409-16.Taipale T, Pienihäkkinen K, Isolauri E, Larsen C, Brockmann E, Alanen P, Jokela J, Söderling E. Bifidobacterium animalis subsp. lactis BB-12 in reducing the risk of infections in infancy. Br J Nutr, 2011 Feb, 105(3): 409-16.Taipale TJ, Pienihäkkinen K, Isolauri E, Jokela JT, Söderling EM. Bifidobacterium animalis subsp. lactis BB-12 in reducing the risk of infections in early childhood. Pediatr Res, 2016 Jan, 79(1-1): 65-9. doi: 10.1038/pr.2015.174.Taipale TJ, Pienihäkkinen K, Isolauri E, Jokela JT, Söderling EM. Bifidobacterium animalis subsp. lactis BB-12 in reducing the risk of infections in early childhood. Pediatr Res, 2016 Jan, 79(1-1): 65-9. doi: 10.1038/pr.2015.174.Rizzardini G1, Eskesen D, Calder PC, Capetti A, Jespersen L, Clerici M. Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. Br J Nutr, 2012 Mar, 107(6): 876-84.Rizzardini G1, Eskesen D, Calder PC, Capetti A, Jespersen L, Clerici M. Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. Br J Nutr, 2012 Mar, 107(6): 876-84.Ouwehand AC, DongLian C, Weijian X, Stewart M, Ni J, Stewart T, et al. Probiotics reduce symptoms of antibiotic use in a hospital setting: a randomized dose response study. Vaccine, 2014 Jan 16, 32(4): 458–63.Ouwehand AC, DongLian C, Weijian X, Stewart M, Ni J, Stewart T, et al. Probiotics reduce symptoms of antibiotic use in a hospital setting: a randomized dose response study. Vaccine, 2014 Jan 16, 32(4): 458–63.Ringel Y, Ringel-Kulka T, Maier D, Carroll I, Galanko JA, Leyer G et al. Probiotic Bacteria Lactobacillus acidophilus NCFM and Bifidobacte rium lactis Bi-07 Versus Placebo for the Symptoms of Bloating in Patients With Functional Bowel Disorders-a Double-blind Study. J Clin Gastroenterol, 2011 Jul, 45(6): 518–525.Ringel Y, Ringel-Kulka T, Maier D, Carroll I, Galanko JA, Leyer G et al. Probiotic Bacteria Lactobacillus acidophilus NCFM and Bifidobacte rium lactis Bi-07 Versus Placebo for the Symptoms of Bloating in Patients With Functional Bowel Disorders-a Double-blind Study. J Clin Gastroenterol, 2011 Jul, 45(6): 518–525.Ringel‐Kulka T, Goldsmith J, Carroll I, Barros S, Palsson O, Jobin C, et al. Lactobacillus acidophilus NCFM affects colonic mucosal opioid receptor expression in patients with functional abdominal pain‐a randomised clinical study. Alimentary pharmacology & therapeutics, 2014, 40(2): 200-7.Ringel‐Kulka T, Goldsmith J, Carroll I, Barros S, Palsson O, Jobin C, et al. Lactobacillus acidophilus NCFM affects colonic mucosal opioid receptor expression in patients with functional abdominal pain‐a randomised clinical study. Alimentary pharmacology & therapeutics, 2014, 40(2): 200-7.Magro DO, de Oliveira LMR, Bernasconi I, de Souza Ruela M, Credidio L, Barcelos IK, et al. Effect of yogurt containing polydextrose, Lactobacillus acidophilus NCFM and Bifidobacterium lactis HN019: a randomized, double-blind, controlled study in chronic constipation. Nutrition journal, 2014, 13(1): 75. doi: 10.1186/1475-2891-13-75.Magro DO, de Oliveira LMR, Bernasconi I, de Souza Ruela M, Credidio L, Barcelos IK, et al. Effect of yogurt containing polydextrose, Lactobacillus acidophilus NCFM and Bifidobacterium lactis HN019: a randomized, double-blind, controlled study in chronic constipation. Nutrition journal, 2014, 13(1): 75. doi: 10.1186/1475-2891-13-75.Sui J, Leighton S, Busta F, Brady L. 16S ribosomal DNA analysis of the faecal lactobacilli composition of human subjects consuming a probiotic strain Lactobacillus acidophilus NCFM. J Appl Microbiol, 2002, 93(5): 907-12.Sui J, Leighton S, Busta F, Brady L. 16S ribosomal DNA analysis of the faecal lactobacilli composition of human subjects consuming a probiotic strain Lactobacillus acidophilus NCFM. J Appl Microbiol, 2002, 93(5): 907-12.Engelbrekston A, Korzenik JR, Pittler A, Sanders ME, Klaenhammer TR, Leyer G, Kitts CL. Pro bio-tics to minimize the disruption of fecal microbiota in healthy subjects undergoing antibiotic therapy. J Med Microbiol, 2009, 58: 633-670.Engelbrekston A, Korzenik JR, Pittler A, Sanders ME, Klaenhammer TR, Leyer G, Kitts CL. Pro bio-tics to minimize the disruption of fecal microbiota in healthy subjects undergoing antibiotic therapy. J Med Microbiol, 2009, 58: 633-670.Doré J, Multon M-Ch, Béhier J-M. The human gut microbiome as source of innovation for health: Which physiological and therapeutic outcomes could we expect? Thérapie, 2017, 72(1): 21-38. http://dx.doi.org/10.1016/j.therap.2016.12.007.Doré J, Multon M-Ch, Béhier J-M. The human gut microbiome as source of innovation for health: Which physiological and therapeutic outcomes could we expect? Thérapie, 2017, 72(1): 21-38. http://dx.doi.org/10.1016/j.therap.2016.12.007.Proctor LM. The National Institutes of Health Human Microbiome Project. Seminars in Fetal & Neonatal Medicine, 2016, 21: 368-372.Proctor LM. The National Institutes of Health Human Microbiome Project. Seminars in Fetal & Neonatal Medicine, 2016, 21: 368-372.Jahns L, Baturin A, Popkin BM. Obesity, diet, and poverty: trends in the Russian transition to market economy. Eur. J. Clin. Nutr., 2003, 57: 1295–1302.Jahns L, Baturin A, Popkin BM. Obesity, diet, and poverty: trends in the Russian transition to market economy. Eur. J. Clin. Nutr., 2003, 57: 1295–1302.

The authors declare that there are no conflicts of interest present.