References

medsovet

Медицинский Совет

Meditsinskiy sovet = Medical Council

2079-701X2658-5790

REMEDIUM GROUP Ltd.

10.21518/2079-701X-2021-21-2-80-85

medsovet-6638

Research Article

Дерматология

Dermatology

Выбор биомаркеров для исследований келоидной ткани после лазерной терапии

Evaluation of biomarkers in the studies of keloid tissue after laser therapy

https://orcid.org/0000-0001-7100-1068

Мезенцев

А. В.

Mezentsev

A. V.

Мезенцев Александр Викторович – кандидат биологических наук, старший научный сотрудник.

109029, Москва, ул. Средняя Калитниковская, д. 30.

Alexandre V. Mezentsev - Cand. Sci. (Biol.), Senior Researcher, Center for Theoretical Problems of Physicochemical Pharmacology RAS.

30, Srednyaya Kalitnikovskaya St., Moscow, 109029.

mesentsev@yahoo.com

https://orcid.org/0000-0002-4862-2779

Карапетян

М. М.

Karapetyan

M. M.

Карапетян Мари Мануковна - врач-дерматовенеролог.

141008, Московская область, Мытищи, ул. Колпакова, д. 41.

Mari M. Karapetyan - Dermatologist, Zone of Smile LLC.

41, Kolpakov St., Mytishchi, Moscow region, 141008.

marimanukovna@mail.ru

https://orcid.org/0000-0003-4779-156X

Соболев

В. В.

Sobolev

V. V.

Соболев Владимир Васильевич - кандидат биологических наук, старший научный сотрудник, НИИВС им. И.И. Мечникова; ЦТП ФХФ РАН.

105064, Москва, Малый Казенный переулок, д. 5а; 109029, Москва, ул. Средняя Калитниковская, д. 30.

Vladimir V. Sobolev - Cand. Sci. (Biol.), Senior Researcher, Mechnikov Scientific and Research Institute of Vaccines and Serums; Center for Theoretical Problems of Physicochemical Pharmacology RAS.

5a, Malyy Kazenny Lane, Moscow, 105064; 30, Srednyaya Kalitnikovskaya St., Moscow, 109029.

vlsobolew@gmail.com

https://orcid.org/0000-0001-5723-6573

Жукова

О. В.

Zhukova

O. V.

Жукова Ольга Валентиновна – доктор медицинских наук, профессор, главный врач.

119071, Москва, Ленинский проспект, д. 17.

Olga V. Zhukova - Dr. Sci. (Med.), Professor, Chief Physician, Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology.

17, Leninskiy Ave., Moscow, 119071.

klinderma@inbox.ru

https://orcid.org/0000-0002-6583-0318

Корсунская

И. М.

Korsunskaya

I. M.

Корсунская Ирина Марковна - доктор медицинских наук, профессор, заведующая лабораторией.

109029, Москва, ул. Средняя Калитниковская, д. 30.

Irina M. Korsunskaya - Dr. Sci. (Med.), Head of Laboratory, Center for Theoretical Problems of Physicochemical Pharmacology RAS.

30, Srednyaya Kalitnikovskaya St., Moscow, 109029.

marykor@bk.ru

Центр теоретических проблем физико-химической фармакологии РАНРоссияCenter for Theoretical Problems of Physicochemical Pharmacology RASRussian Federation

ООО «Зона улыбки»РоссияZone of Smile LLCRussian Federation

Центр теоретических проблем физико-химической фармакологии РАН; Научно-исследовательский институт вакцин и сывороток имени И.И. МечниковаРоссияCenter for Theoretical Problems of Physicochemical Pharmacology RAS; Mechnikov Scientific and Research Institute of Vaccines and SerumsRussian Federation

Московский научно-практический центр дерматовенерологии и косметологииРоссияMoscow Scientific and Practical Center of Dermatovenereology and CosmetologyRussian Federation

2021

09012022

021-28085

2022

Мезенцев А.В., Карапетян М.М., Соболев В.В., Жукова О.В., Корсунская И.М.

Mezentsev A.V., Karapetyan M.M., Sobolev V.V., Zhukova O.V., Korsunskaya I.M.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.med-sovet.pro/jour/article/view/6638

В настоящем исследовании обсуждаются принципы подбора биомаркеров для описания результатов лечения келоидной ткани методом лазерной терапии. В работе представлен краткий обзор основных цитомаркеров (Krt14, Lgals7, Krt5, Dcn, Lum, Igfbp5, Cd31, Vwf, Stambpl1, Uqcrb, Cd3 и Acta2), биомаркеров воспалительного процесса (Cd45/Ptprc, Adgre1, Ly6g, Il1b, Il4, Il13, Il22, Cxcl2 и Ccl17), а также белковых компонентов межклеточного матрикса (коллагенов I и III типа, прекурсоров COL5A1 и COLA1A, FTL, COL3A1, PGLS, CNN2, ANXA2, TPSAB1, COL12A1, прекурсоров APCS и ALB) и кодирующих белки межклеточного матрикса генов (FGF7, BAX, CCND1, MMP3, MMP9, CXCL1, -2, -5, -6 и -12, IL8, S100A7 и IL1A), содержание и взаимное расположение которых может потенциально привести к изменениям внутренней структуры и внешнего облика пораженного участка кожи. Нами также описаны процесс подбора биомаркеров по результатам полногеномных исследований и связанные с их выбором ограничения. Помимо этого, мы приводим конкретные примеры применения различных групп генных и белковых маркеров в экспериментальной и клинической практике. Согласно приводимым в работе данным литературы, уже установленные и опробованные на лабораторных моделях группы биомаркеров в зависимости от своих функциональных возможностей позволяют описать структурные и цитологические аномалии пораженных участков кожи, причем как до, так и после терапевтического воздействия. Кроме того, имеющиеся экспериментальные и клинические данные делают возможным анализ эффективности новых экспериментальных подходов, а также сравнение результатов нескольких уже проведенных исследований.

In this paper, we discuss what biomarkers to choose if there is a need to describe the results of laser therapy targeting keloid skin. We elevate the known cytomarkers (Krt14, Lgals7, Krt5, Dcn, Lum, Igfbp5, Cd31, Vwf, Stambpl1, Uqcrb, Cd3 and Acta2), biomarkers of the inflammatory response (Cd45/Ptprc, Adgre1, Ly6g, Il1b, Il4, Il13, Il22, Cxcl2 и Ccl17), as well as the proteins of extracellular matrix (type I and III collagens; precursors of COL5A1 and COLA1A; FTL, COL3A1, PGLS, CNN2, ANXA2, TPSAB1, COL12A1, precursors of APCS and ALB), and their encoding genes (FGF7, BAX, CCND1, MMP3, MMP9, CXCL1, -2, -5, -6 and -12; IL8, S100A7 and IL1A), those expression and co-location may potentially change the appearance and internal structure of damaged skin. We also describe how to choose biomarkers using the results genomic studies and their limitations. Moreover, we provide examples of how different groups of gene and protein biomarkers are used in experimental biology and clinical practice. According to the previously published data, well-known biomarkers verified on animal models, depend on their biological effects, let to characterize structural changes and changes in the composition of cells represented at the site of damage before and after the treatment. In addition, the published experimental and clinical data provide an opportunity to analyze the efficiency of new experimental approaches and compare them to each other.

фиброз кожилазерная терапиягены-маркерыцитомаркерымолекулярная сигнатурадифференциальная экспрессия генов

skin fibrosislaser therapygene biomarkerscytomarkersmolecular signaturedifferential gene expression

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The authors declare that there are no conflicts of interest present.