medsovetМедицинский СоветMeditsinskiy sovet = Medical Council2079-701X2658-5790REMEDIUM GROUP Ltd.10.21518/2079-701X-2022-16-1-65-75medsovet-6686Research ArticleБРОНХОПУЛЬМОНОЛОГИЯ, ОТОРИНОЛАРИНГОЛОГИЯBRONCHOPULMONOLOGY, OTORHINOLARYNGOLOGYКлинико-микробиологическое обоснование использования растительного препарата у детей с ринофарингитомClinical and microbiological rationale for the use of a herbal preparation in children with rhinopharyngitishttps://orcid.org/0000-0002-6719-5805ЧелпаченкоО. Е.ChelpachenkoO. E.

 д.м.н., профессор, ведущий научный сотрудник лаборатории биомониторинга и молекулярно-генетических исследований

 460000, Россия, Оренбург, ул. Пионерская, д. 11 

 Dr. Sci. (Med.), Leading Researcher, Laboratory of Biomonitoring and Molecular Genetic Research

11, Pionerskaya St., Orenburg, 460000, Russia 

oech57@gmail.comhttps://orcid.org/0000-0003-0910-6525ДаниловаЕ. И.DanilovaE. I.

 к.м.н., доцент кафедры педиатрии 

 460000, Россия, Оренбург, ул. Советская, д. 6 

 Cand. Sci. (Med.), Associate Professor of the Department of Pediatrics

6, Sovetskaya St., Orenburg, 460000, Russia 

danilowa@list.ruhttps://orcid.org/0000-0002-8923-8829ЧайниковаИ. Н.ChainikovaI. N.

 д.м.н., профессор, ведущий научный сотрудник лаборатории биомониторинга и молекулярно-генетических исследований; профессор кафедры нормальной физиологии

460000, Россия, Оренбург, ул. Пионерская, д. 11

460000, Россия, Оренбург, ул. Советская, д. 6 

 Dr. Sci. (Med.), Professor, Leading Researcher, Laboratory for Biological Monitoring and Molecular Genetic Research; Professor of the Department of Normal Physiology

11, Pionerskaya St., Orenburg, 460000, Russia

6, Sovetskaya St., Orenburg, 460000, Russia 

inchainicova@yandex.ruhttps://orcid.org/0000-0001-5020-2493БекпергеноваА. В.BekpergenovaA. V.

 к.б.н., научный сотрудник лаборатории биомониторинга и молекулярно-генетических исследований 

 460000, Россия, Оренбург, ул. Пионерская, д. 11 

 Cand. Sci. (Biol.), Laboratory for Biological Monitoring and Molecular Genetic Research

11, Pionerskaya St., Orenburg, 460000, Russia 

nsavasteeva@gmail.comhttps://orcid.org/0000-0001-5186-6865БондаренкоТ. А.BondarenkoT. A.

 научный сотрудник лаборатории биомониторинга и молекулярно-генетических исследований 

 460000, Россия, Оренбург, ул. Пионерская, д. 11 

 Researcher, Laboratory for Biological Monitoring and Molecular Genetic Research

11, Pionerskaya St., Orenburg, 460000, Russia 

semenovih88@gmail.comhttps://orcid.org/0000-0002-7320-7331СуменкоВ. В.SumenkoV. V.

 к.м.н., доцент кафедры педиатрии Института последипломного, образования 

 460000, Россия, Оренбург, ул. Советская, д. 6 

 Cand. Sci. (Med.), Associate Professor of the Department of Pediatrics, Institute of Postgraduate Training

6, Sovetskaya St., Orenburg, 460000, Russia 

sumenkovv@mail.ruИнститут клеточного и внутриклеточного симбиоза Уральского отделения РАНРоссияInstitute of Cellular and Intracellular Symbiosis, Ural Branch of RASRussian FederationОренбургский государственный медицинский университетРоссияInstitute of Postgraduate Training, Orenburg State Medical UniversityRussian FederationИнститут клеточного и внутриклеточного симбиоза Уральского отделения РАН; Оренбургский государственный медицинский университетРоссияInstitute of Cellular and Intracellular Symbiosis, Ural Branch of RAS; Orenburg State Medical UniversityRussian FederationОренбургский государственный медицинский университетРоссияOrenburg State Medical UniversityRussian Federation202227022022016575Copyright © Челпаченко О.Е., Данилова Е.И., Чайникова И.Н., Бекпергенова А.В., Бондаренко Т.А., Суменко В.В., 20222022Челпаченко О.Е., Данилова Е.И., Чайникова И.Н., Бекпергенова А.В., Бондаренко Т.А., Суменко В.В.Chelpachenko O.E., Danilova E.I., Chainikova I.N., Bekpergenova A.V., Bondarenko T.A., Sumenko V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-sovet.pro/jour/article/view/6686

Введение. Работа включает результаты клинико-микробиологических исследований, определяющих целесообразность использования растительного препарата EPS® 7630 для лечения ринофарингита (РФ) у детей. Во введении представлен краткий обзор литературных данных о роли микробного фактора и важном вкладе дисбиотических нарушений микрофлоры верхних дыхательных путей в развитие РФ. Обозначена роль персистентных свойств условно-патогенных микроорганизмов (УПМ): способность к биопленкообразованию (БПО) и инактивация лизоцима (антилизоцимная активность – АЛА) в качестве дисбиотических маркеров РФ, которые создают условия для рецидивов и хронизации заболевания, снижают эффективность антимикробной терапии.Цель работы – доказать клинико-микробиологическое обоснование эффективности использования EPS® 7630 в лечении РФ у детей.Материалы и методы. В исследовании участвовали 79 детей в возрасте от 3 до 15 лет: 1-я группа – 43 ребенка, которым проводилось лечение, включающее, наряду с традиционным лечением, препарат EPS® 7630, 2-я группа (сравнения) – 36 детей, не получавших тестируемый препарат. Микробиологическое исследование проводилось путем взятия мазков из зева и носа до и после лечения; БПО и АЛА определяли по методикам О.В. Бухарина и др. (1999).Результаты. Проведенные клинические исследования установили сокращение сроков купирования основных симптомов РФ (кашель, ринит, боли в горле, лихорадка) у детей, получавших лечение EPS® 7630 относительно группы сравнения. Микробиологические исследования микроорганизмов, выделенных из носоглотки детей с РФ, выявили способность EPS® 7630 подавлять факторы персистенции бактерий (БПО и АЛА) на 60% и более от исходного уровня.Выводы. Полученные данные раскрывают один из механизмов антимикробного действия EPS® 7630, заключающийся в подавлении персистентного потенциала УПМ и регулирующем влиянии препарата на микробиоценоз верхних дыхательных путей. Доказана клиническая и микробиологическая эффективность использования EPS® 7630 при лечении РФ у детей.

Introduction

Introduction. The work includes the results of clinical and microbiological studies that determine the feasibility of using the herbal preparation EPS® 7630 for the treatment of rhinopharyngitis (RF) in children. The introduction provides a brief review of the literature data on the role of the microbial factor and the important contribution of dysbiotic disorders of the URT microflora in the development of the Russian Federation. The porpose of persistent properties of opportunistic microorganisms (UPM) is outlined: the ability to biofilm formation – BPO and inactivation of lysozyme – ALA as dysbiotic markers of RF, which create conditions for relapses and chronicity of the disease, reduce the effectiveness of antimicrobial therapy. Goal.

The aim of the work was the clinical and microbiological substantiation of the effectiveness of using EPS® 7630 in the treatment of RF in children.

Materials and methods

Materials and methods. The study involved 79 children aged 3 to 15 years. 1st group – 43 children who received treatment, including, along with traditional treatment, the drug Umckalor EPS® 7630, 2nd group (comparison) – 36 children who did not receive the tested drug. Microbiological examination was carried out by taking swabs from the pharynx and nose before and after treatment; BPO and ALA were determined according to the methods of Bukharin O.V. et al. (1999).

Results

Results. Conducted clinical studies have established a reduction in the timing of relief of the main symptoms of RF (cough, rhinitis, sore throat, fever) in children treated with EPS® 7630 compared to the comparison group. Microbiological studies of microorganisms isolated from the nasopharynx of children with RF revealed the ability of EPS® 7630 to suppress bacterial persistence factors (BPO and ALA) by 60% or more from the initial level.

Conclusions

Conclusions. The data obtained reveal one of the mechanisms of the antimicrobial action of EPS® 7630, which consists in suppressing the persistent potential of UPM and regulating the effect of the drug on the microbiocenosis of the URT. The clinical and microbiological efficacy of using EPS® 7630 in the treatment of RF in children has been proven.

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The authors declare that there are no conflicts of interest present.