medsovetМедицинский СоветMeditsinskiy sovet = Medical Council2079-701X2658-5790REMEDIUM GROUP Ltd.10.21518/ms2024-136medsovet-8291Research ArticleПРИКЛАДНЫЕ АСПЕКТЫ ЭНДОКРИНОЛОГИИ И КАРДИОЛОГИИAPPLIED ASPECTS OF ENDOCRINOLOGY AND CARDIOLOGYМетаболическая активность микробиоты толстой кишки у пациентов с ожирением (пилотное исследование)Metabolic activity of the colon microbiota in patients with obesity (pilot research)https://orcid.org/0000-0002-6329-593XДушинаТ. С.DushinaT. S.

Душина Татьяна Сергеевна, ассистент кафедры терапии с курсами эндокринологии, функциональной и ультразвуковой диагностики Института клинической медицины, Тюменский государственный медицинский университет

625023, Тюмень, ул. Одесская, д. 54

Tatyana S. Dushina, Assistant of the Department of the Therapy with courses in Endocrinology, Functional and Ultrasound Diagnostics

54, Odesskaya St., Tyumen, 625023

dr.dushina@mail.ruhttps://orcid.org/0000-0001-9253-8075СуплотоваЛ. А.SuplotovaL. A.

Суплотова Людмила Александровна, д.м.н., профессор, заведующая курсом эндокринологии кафедры терапии

625023, Тюмень, ул. Одесская, д. 54

Lyudmila A. Suplotova, Dr. Sci. (Med.), Professor, Head of the Endocrinology course of the Institute of CPD with the courses of Endocrinology,Functional and Ultrasound Diagnostics

54, Odesskaya St., Tyumen, 625023

suplotovala@mail.ruhttps://orcid.org/0000-0001-7739-3859КляшевС. М.KlyashevS. M.

Кляшев Сергей Михайлович, д.м.н., профессор, заведующий кафедрой терапии с курсами эндокринологии, функциональной и ультразвуковой диагностики Института клинической медицины

625023, Тюмень, ул. Одесская, д. 54

Sergey M. Klyashev, Dr. Sci. (Med.), Professor, Head of Department of Therapy with courses in Endocrinology, Functional and Ultrasound Diagnostics

54, Odesskaya St., Tyumen, 625023

tsma.ks@yandex.ruhttps://orcid.org/0009-0005-3397-9264ФедосееваН. Н.FedoseevaN. N.

Федосеева Наталья Николаевна, к.м.н., доцент кафедры терапии с курсами эндокринологии, функциональной и ультразвуковой диагностики Института клинической медицины

625023, Тюмень, ул. Одесская, д. 54

Natalia N. Fedoseeva, Cand. Sci. (Med.), Assistant Professor of the Department of the Therapy with courses in Endocrinology, Functional andUltrasound Diagnostics

54, Odesskaya St., Tyumen, 625023

nataly.fed@mail.ruТюменский государственный медицинский университетРоссияTyumen State Medical UniversityRussian Federation20242005202406217225Copyright © Душина Т.С., Суплотова Л.А., Кляшев С.М., Федосеева Н.Н., 20242024Душина Т.С., Суплотова Л.А., Кляшев С.М., Федосеева Н.Н.Dushina T.S., Suplotova L.A., Klyashev S.M., Fedoseeva N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-sovet.pro/jour/article/view/8291Введение

Введение. Глобальное экономическое и социальное бремя ожирения требует четкого понимания причин и основных факторов, способствующих его развитию, что позволит разработать и эффективно реализовать потенциальные терапевтические пути для непосредственного воздействия на патологию. В последнее время в патогенезе ожирения все большее значение придается состоянию и разнообразию кишечной микробиоты, основное взаимодействие которой с человеком осуществляется через короткоцепочечные жирные кислоты (КЦЖК) – биологически активные вещества, образующиеся при анаэробной ферментации неперевариваемых углеводов.

Цель

Цель. Оценить метаболическую активность микробиоты толстой кишки по количеству и доле уровней короткоцепочечных жирных кислот у молодых пациентов с ожирением с учетом степени ожирения в сравнении со здоровыми лицами.

Материалы и методы

Материалы и методы. В исследовании приняли участие 87 пациентов с ожирением и 31 человек с нормальной массой тела. Все участники исследования соответствовали критериям включения и подписали информированное согласие. Помимо заполнения анкеты, специально разработанной под цели и задачи данного исследования, проведены антропометрические измерения и определен уровень КЦЖК в фекалиях методом газожидкостной хроматографии на оборудовании «Хромос» ГХ-1000 в независимой лаборатории INVITRO.

Результаты

Результаты. У молодых пациентов с ожирением отмечается более высокая концентрация КЦЖК и изоформ КЦЖК в фекалиях, по сравнению со здоровыми лицами, при этом статистически значимо выше в группе пациентов с ИМТ более 40 кг/м2. Доля ацетата была статистически значимо выше в группе пациентов с нормальным весом, в то время как доля пропионата была выше в группе пациентов с ожирением. В отношении доли бутирата статистических различий получено не было. Кроме того, были выявлены ассоциации КЦЖК с антропометрическими параметрами.

Выводы

Выводы. Результаты исследования подтверждают возможную роль КЦЖК в патогенезе ожирения.

Introduction

Introduction. The global economic and social burden of obesity requires a clear understanding of the causes and underlying factors contributing to its development which will allow to develop and implement effectively the potential therapeutic pathways to direct influence on the pathology. Recently, in the pathogenesis of obesity, great attention is paid to the state and diversity of the intestinal microbiota, its main interaction with men is performed through short-chain fatty acids (SCFAs) – biologically active substances being formed during the anaerobic fermentation of indigestible carbohydrates.

Aim

Aim. To assess the metabolic activity of the colon microbiota, in terms of quantity and proportion of short-chain fatty acid levels in young obese patients taking into account the degree of obesity in comparison with healthy individuals.

Materials and methods

Materials and methods. 87 obese patients and 31 subjects with the normal body weight were included in the study. All study participants met the inclusion criteria and signed the informed consent. In addition to filling out a questionnaire specially designed for the goals and objectives of this study, anthropometric measurements were carried out and the level of SCFAs in feces was determined with the help of gas-liquid chromatography usingChromos GC-1000 equipment in the independent INVITRO laboratory.

Results

Results. Young obese patients have a higher concentration of SCFAs and SCFA isoforms in feces compared to healthy individuals, while it is statistically significantly higher in the group of patients with BMI more than 40 kg/m2. The proportion of acetate was statistically significantly higher in the normal weight group, while the proportion of propionate was higher in the obese group. No statistical differences were found regarding the proportion of butyrate. In addition, the associations of SCFAs with anthropometric parameters were identified.

Conclusion

Conclusion. The results of the study confirm the possible role of SCFA in in pathogenesis of obesity.  

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The authors declare that there are no conflicts of interest present.