medsovetМедицинский СоветMeditsinskiy sovet = Medical Council2079-701X2658-5790REMEDIUM GROUP Ltd.10.21518/ms2025-007medsovet-8942Research ArticleЗАБОЛЕВАНИЯ КОЖИDERMAL DISEASESИсследование профиля экспрессии секретируемых матриксных металлопротеиназ в коже, пораженной склеродермиейSecreted matrix metalloproteinases: Study of the expression profiles in the skin affected by sclerodermahttps://orcid.org/0000-0002-4972-9922МезенцевА. В.MezentsevA. V.

Мезенцев Александр Викторович, к.б.н., ведущий научный сотрудник

109029, Москва, ул. Средняя Калитниковская, д. 30

Alexandre V. Mezentsev, Cand. Sci. (Biol.), Leading Researcher

30, Srednyaya Kalitnikovskaya St., Moscow, 109029

mesentsev@yahoo.comhttps://orcid.org/0000-0002-5913-9635НевозинскаяЗ. А.NevozinskayaZ. A.

Невозинская Зофия Анатольевна, к.м.н., врач-дерматовенеролог

119071, Москва, Ленинский проспект, д. 17

Zofia A. Nevozinskaya, Cand. Sci. (Med.), Dermatovenerologist

20, Seleznevskaya St., Moscow, 127473

nezosia@mail.ruhttps://orcid.org/0000-0003-4779-156XСоболевВ. В.SobolevV. V.

Соболев Владимир Васильевич, к.б.н., старший научный сотрудник

109029, Москва, ул. Средняя Калитниковская, д. 30

Vladimir V. Sobolev, Cand. Sci. (Biol.), Senior Researcher

30, Srednyaya Kalitnikovskaya St., Moscow, 109029

vlsobolew@gmail.comhttps://orcid.org/0000-0002-9158-1933СоболеваА. Г.SobolevaA. G.

Соболева Анна Геннадьевна, к.б.н., старший научный сотрудник, Центр теоретических проблем физико-химической фармакологии Российской академии наук; научный сотрудник, Научно-исследовательский институт морфологии человека имени академика А.П. Авцына Российского научного центра хирургии имени академика Б.В. Петровского

109029, Москва, ул. Средняя Калитниковская, д. 30,

117418, Москва, ул. Цюрупы, д. 3

Anna G. Soboleva, Cand. Sci. (Biol.), Senior Researcher, Center for Theoretical Problems of Physical and Chemical Pharmacology of Russian Academy of Sciences; Researcher, Avtsyn Research Institute of Human Morphology of Petrovsky National Research Centre of Surgery

30, Srednyaya Kalitnikovskaya St., Moscow, 109029,

3, Tsyurupa St., Moscow, 117418

annasobo@mail.ruhttps://orcid.org/0000-0002-9578-5490ПотекаевН. Н.PotekaevN. N.

Потекаев Николай Николаевич, д.м.н., профессор, заведующий кафедрой кожных болезней и косметологии факультета дополнительного профессионального образования, Российский национальный исследовательский медицинский университет имени Н.И. Пирогова; директор, Московский научно-практический центр дерматовенерологии и косметологии

117997, Москва, ул. Островитянова, д. 1,

119071, Москва, Ленинский проспект, д. 17

Nikolay N. Potekaev, Dr. Sci. (Med.), Professor, Head of the Department of Skin Diseases and Cosmetology, Faculty of Additional Professional Education, Pirogov Russian National Research Medical University; Director, Moscow Scientific and Practical Center of Dermatovenereology and Cosmetology

1, Ostrovityanov St., Moscow, 117997,

17, Leninskiy Ave., Moscow, 119071

klinderma@mail.ruhttps://orcid.org/0000-0002-6583-0318КорсунскаяИ. М.KorsunskayaI. M.

Корсунская Ирина Марковна, д.м.н., профессор, заведующая лабораторией физико-химических и генетических проблем дерматологии

109029, Москва, ул. Средняя Калитниковская, д. 30

Irina M. Korsunskaya, Dr. Sci. (Med.), Professor, Head of the Laboratory of Physicochemical and Genetic Problems in Dermatology

30, Srednyaya Kalitnikovskaya St., Moscow, 109029

marykor@bk.ruЦентр теоретических проблем физико-химической фармакологии Российской академии наукРоссияCenter for Theoretical Problems of Physical and Chemical Pharmacology of Russian Academy of SciencesRussian FederationМосковский научно-практический центр дерматовенерологии и косметологииРоссияMoscow Scientific and Practical Center of Dermatovenereology and CosmetologyRussian FederationЦентр теоретических проблем физико-химической фармакологии Российской академии наук; Научно-исследовательский институт морфологии человека имени академика А.П. Авцына Российского научного центра хирургии имени академика Б.В. ПетровскогоРоссияCenter for Theoretical Problems of Physical and Chemical Pharmacology of Russian Academy of Sciences; Avtsyn Research Institute of Human Morphology of Petrovsky National Research Centre of SurgeryRussian FederationМосковский научно-практический центр дерматовенерологии и косметологии; Российский национальный исследовательский медицинский университет имени Н.И. ПироговаРоссияMoscow Scientific and Practical Center of Dermatovenereology and Cosmetology; Pirogov Russian National Research Medical UniversityRussian Federation20253003202502815Copyright © Мезенцев А.В., Невозинская З.А., Соболев В.В., Соболева А.Г., Потекаев Н.Н., Корсунская И.М., 20252025Мезенцев А.В., Невозинская З.А., Соболев В.В., Соболева А.Г., Потекаев Н.Н., Корсунская И.М.Mezentsev A.V., Nevozinskaya Z.A., Sobolev V.V., Soboleva A.G., Potekaev N.N., Korsunskaya I.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-sovet.pro/jour/article/view/8942Введение

Введение. Локализованная склеродермия – редкое аутоиммунное заболевание, при котором происходит уплотнение отдельных участков кожи вследствие локального накопления белков дермального межклеточного матрикса, главным образом коллагена. Матриксные металлопротеиназы (протеолитические ферменты) – основные участники катаболизма коллагена, отвечающие за поддержание постоянства белкового состава дермиса. Изменения в активности и нарушения в экспрессии матриксных металлопротеиназ могут стать причиной развития болезни.

Цель

Цель. Оценить изменения в экспрессии секретируемых матриксных металлопротеиназ (ММП-1, ММП-2, ММП-3 и ММП-9) в пораженной склеродермией коже с перспективой разработки таргетных препаратов, используя метод количественной полимеразной цепной реакции.

Материалы и методы

Материалы и методы. В работе исследовали биоптаты больных склеродермией, которые были получены из пораженной и непораженной болезнью кожи. Для оценки изменений генной экспрессии использовали метод полимеразной цепной реакции.

Результаты

Результаты. При анализе экспериментальных данных установлено увеличение экспрессии ММП-2 (3,379 ± 1,177) и ММП-9 (4,471 ± 1,836), а также снижение экспрессии ММП-1 (0,169 ± 0,036) и ММП-3 (0,240 ± 0,086). Показана строгая отрицательная корреляция (r = –0,93) между уровнем экспрессии ММП-1 и площадью поражения кожи. При проведении компьютерного анализа установлена взаимосвязь между изменениями в экспрессии ММП и особенностями поражения кожи.

Выводы

Выводы. Результаты работы подтверждают возможность использования ММП в качестве биомаркеров для оценки степени тяжести склеродермии и перспективного объекта (молекулярной мишени) для терапевтического вмешательства.

Introduction

Introduction. Localized scleroderma is a rare autoimmune disorder characterized by a thickening of the skin due to the overdeposition of collagens and other proteins in the dermal extracellular matrix. Matrix metalloproteinases (MMPs) is a family of proteolytic enzymes, playing a crucial role in the catabolism of collagens, maintaining the protein composition of the dermis. Alterations in MMPs activity and expression contribute to the development of the disease.

Aim

Aim. To investigate changes in the expression of secreted matrix metalloproteinases (MMP-1, MMP-2, MMP-3, and MMP-9) in scleroderma lesional skin using quantitative polymerase chain reaction to identify potential therapeutic targets.

Materials and methods

Materials and methods. Biopsies of lesional and unaffected skin were obtained from patients with scleroderma. PCR was used to assess changes in the expression of MMPs.

Results

Results. The analysis of experimental data revealed significant changes in the expression of MMPs: the increased levels of MMP-2 (3.379 ± 1.177) and MMP-9 (4.471 ± 1.836), and the decreased levels of MMP-1 (0.169 ± 0.036) and MMP-3 (0.240 ± 0.086).

The correlation analysis revealed a strong negative correlation (r = –0.93) between the expression of MMP-1 and the area of scleroderma lesional skin. The computer analysis suggested that changes in the expression of MMPs may contribute to the development of skin lesions as a factor.

Conclusions

Conclusions. The results of this study suggest that some MMPs may serve as biomarkers for assessing the severity of localized scleroderma and as potential molecular targets for therapeutic intervention.

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The authors declare that there are no conflicts of interest present.