Vaginal microbiota in bacterial vaginosis. Aspects of diagnosis and therapy
https://doi.org/10.21518/2079-701X-2014-9-90-95
Abstract
Bacterial vaginosis is a polymicrobial clinical syndrome characterized by the replacement of physiological Lactobacillus species with a spectrum of other microorganisms, mostly anaerobic. Numerous studies showed that key components of the vaginal microbiota in BV are Gardnerella vaginalis and Atopobium vaginae. A. vaginae are hard-to-culture bacteria whose involvement in BV has been described recently with the help of molecular methods. The article demonstrates that A. vaginae and G. vaginalis are responsible for the formation of biofilms on the vaginal epithelium. BV has a high recurrence rate, the main causes of which are presumably the resistance of BV-associated bacteria to antimicrobial agents and low susceptibility of biofilms to antibiotics.
About the Authors
A. M. Savicheva
D. O. Ott Research Institute of Obstetrics and Gynecology
Russian Federation
Russian Federation
E. V. Shipitsyna
D. O. Ott Research Institute of Obstetrics and Gynecology
Russian Federation
Russian Federation
References
1. Schwebke JR, Weiss HL. Interrelationships of bacterial vaginosis and cervical inflammation. Sex Transm Dis, 2002, 29: 59-64.
2. Wiesenfeld HC, Hillier SL, Krohn MA, et al. Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin Infect Dis, 2003, 36: 663-8.
3. Allsworth JE, Peipert JF. Severity of bacterial vaginosis and the risk of sexually transmitted infection. Am J Obstet Gynecol, 2011, 205 (2): 113.e1-6.
4. Cherpes TL, Meyn LA, Krohn MA et al. Association between acquisition of herpes simplex virus type 2 in women and bacterial vaginosis. Clin Infect Dis, 2003, 37: 319-25.
5. Myer L, Kuhn L, Stein ZA et al. Intravaginal practices, bacterial vaginosis, and women's susceptibility to HIV infection: epidemio-logical evidence and biological mechanisms. Lancet Infect Dis, 2005, 5: 786-94.
6. Sweet RL Role of bacterial vaginosis in pelvic inflammatory disease. Clin Infect Dis, 1995, 20 (Suppl. 2): S271-275.
7. Ralph SG, Rutherford AJ, Wilson JD. Influence of bacterial vaginosis on conception and miscarriage in the first trimester: cohort study. BMJ, 1999, 319: 220-3.
8. Hay PE, Lamont RF, Taylor-Robinson D et al. Abnormal bacterial colonisation of the genital tract and subsequent preterm delivery and late miscarriage. BMJ, 1994, 308: 295-8.
9. Hillier SL, Nugent RP, Eschenbach DA et al. Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant. The Vaginal Infections and Prematurity Study Group. N Engl J Med, 1995. 333: 1737-42.
10. Silver HM, Sperling RS, St Clair PJ et al. Evidence relating bacterial vaginosis to intraamniotic infection. Am J Obstet Gynecol, 1989. 161: 808-12.
11. Martius J, Eschenbach DA. The role of bacterial vaginosis as a cause of amniotic fluid infection, chorioamnionitis and prematurity - a review. Arch Gynecol Obstet, 1990, 247: 1-13.
12. Watts DH, Krohn MA, Hillier SL et al. Bacterial vaginosis as a risk factor for post-cesarean endometritis. Obstet Gynecol, 1990, 75: 52-8.
13. Hay P. Recurrent bacterial vaginosis. Curr Infect Dis Rep, 2000. 2: 506-512.
14. Larsson PG, Forsum U. Bacterial vaginosis, a disturbed bacterial flora and treatment enigma. APMIS, 2005, 113: 305-316.
15. Lamont RF, Sobel JD, Akins RA et al. The vaginal microbiome: new information about genital tract flora using molecular based techniques. BJOG, 2011, 118: 533-549.
16. Spiegel CA. Bacterial vaginosis. Rev Med Microbiol, 2002. 13: 43-51.
17. Fredricks DN, Fiedler TL, Marrazzo JM. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med, 2005, 353: 1899-911.
18. Verhelst R, Verstraelen H, Claeys G et al. Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol, 2004, 4: 16.
19. Menard JP et al. High vaginal concentrations of Atopobium vaginae and Gardnerella vaginalis in women undergoing preterm labor. Obstet Gynecol, 2010, 115: 134-40.
20. Ferris MJ, Masztal A, Aldridge KE et al. Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis. BMC Infect Dis, 2004, 4: 5.
21. Rodriguez Jovita M, Collins MD, Sjoden B et al. Characterization of a novelAtopobium isolate from the human vagina: description of Atopobium vaginae sp. nov. Int J Syst Bacteriol, 1999, 49 Pt 4: 1573-6.
22. Donati L, Di Vico A, Nucci M et al. Vaginal microbial flora and outcome of pregnancy. Arch Gynecol Obstet, 2010, 281: 589-600.
23. Vestraelen H, Verhelst R, Claeys G et al. Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis. Am J Obstet Gynecol, 2004, 191: 1130-2.
24. Swidsinki A, Mendling W, Loening-Baucke V et al. Adherent biofilms in bacterial vaginosis. Obstet Gynecol, 2005, 106: 1013-23.
25. Watnick P, Kolter R. Biofilm, city of microbes. J Bacteriol, 2000, 182: 2675-9.
26. Verstraelen H, Swidsinski A. The biofilm in bacterial vaginosis: implications for epidemiology, diagnosis and treatment. Curr Opin Infect Dis, 2013, 26(1): 86-9.
27. Alves P, Castro J, Sousa C et al. Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV patients in an in vitro biofilm formation model. J Infect Dis, 2014 [Epub ahead of print].
Review
For citations:
Savicheva AM, Shipitsyna EV. Vaginal microbiota in bacterial vaginosis. Aspects of diagnosis and therapy. Meditsinskiy sovet = Medical Council. 2014;(9):90-95. (In Russ.) https://doi.org/10.21518/2079-701X-2014-9-90-95
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