Histological examination of the palatine tonsils in chronic tonsillitis

The article is devoted to the problem of chronic tonsillitis. The aim of the study was to assess changes in the morphofunctional state of the palatine tonsils in chronic inflammation. The study presents comparative data of immunohistochemical examination of the tonsils in normal and chronic tonsillar pathology. The authors used the clinical classification of chronic tonsillitis developed by B.S. Preobrazhensky and V.T. Pal’chun. Histological changes in the palatine tonsils of patients with toxic-allergic form 2 chronic tonsillitis were described in accordance with this classification. The control group represented palatine tonsils tissue taken from patients without chronic tonsillitis. The examination was carried out using markers of apoptosis, antiapoptosis, cell proliferation, as well as various types of lymphocytes. The authors describe a tonsil tissue handling technique, morphological and functional state in each study group, compared the studied parameters in two groups, reflected the dynamics of changes in the palatine tonsils with chronic tonsillitis. Increased apoptotic and anti-apoptotic activity combined with decreased cell proliferation, increased number of T-helpers and B-lymphocytes gives evidence of significantly decreased functional activity of the palatine tonsils in chronic inflammation. These changes are correlated with the clinical picture to develop the most optimal management of the disease.

chronic tonsillitis, immunohistochemistry, proliferation, apoptosis, antiapoptosis, T-lymphocytes, B-lymphocytes

1. Pal’chun VT. Classification and therapeutic strategy for chronic tonsillitis. Vestnik otorinolaringologii = Bulletin of Otorhinolaryngology. 2013;78(3):8-11. (In Russ.) Available at: https://www.mediasphera. ru/issues/vestnik-otorinolaringolog ii/2013/3/030042-4668201332.

2. Bhattachatyya N., Kepnes L.J. Economic Benefit of Tonsillectomy in Adults with Chronic Tonsillitis. Annals of Otology, Rhinology & Laryngology. 2002;111(11):983-988. doi 10.1177/000348940211101106.

3. Pal’chun V.T., Gurov A.V., Guseva O.A. The specific pathogenetic features of the development of chronic tonsillar pathology. Vestnik otorinolaringologii = Bulletin of Otorhinolaryngology. 2018;83(2):30-33. (In Russ.) doi: 10.17116/otorino201883230-33.

4. Preobrazhenkskiy B.S., Popova G.N. Angina, chronic tonsillitis, related general diseases. Moscow: Medicine; 1970. (In Russ.)

5. Lange M.J., Lasiter J.C., Misfeldt M.L. Toll-like receptors in tonsillar epithelial cells. International Journal of Pediatric Otorhinolaryngology. 2009;73(4):613-621. doi: 10.1016/j.ijporl.2008.12.013.

6. Avramović V., Petrović V., Jović M., Vlahović P. Quantification of cells expressing markers of proliferation and apoptosis in chronic tonsilitis. Acta Otorhinolaryngologica Italica. 2015;35(4):277-84. Available at: https://www. ncbi.nlm.nih.gov/pmc/articles/PMC4731890/.