The associations between rs35089892 CAMK2B gene and atrial fibrillation in a case–control study

Introduction. Atrial fibrillation (AF) is a burden of aging and a common heart rhythm disorder in adults. Predictors of AF remain poorly understood, and arrhythmia is often first diagnosed during or after a cardiovascular event, despite improved diagnostic capabilities. This underscores the relevance of the search for new predictors of cardiovascular risk, including genetic ones.

Aim. To analyze the association between rs35089892 (CAMK2B) and incident AF in subjects aged 45–69 years at the time of inclusion in the study over a13-year follow-up of the cohort.

Materials and methods. The study object was the cohort of Novosibirsk inhabitants, the HAPIEE project (n = 9,360; baseline examination 2003–2005), follow-up period to 01/31/2018 was analyzed. Among participants of the cohort with incident AF (n = 473), a “case” group (n = 65; 45% of men) was selected. The “control” group (n = 65) – individuals without arrhythmia, stratified by sex and age. AF was determined by ECG or documented AF cases. Genotyping of rs35089892 (CAMK2B) was performed using the polymerase chain reaction. Statistical data processing was carried out by SPSS.

Results. The carriage of TT genotype rs35089892 was inversely associated with the risk of incident AF (p = 0.003) and the relationship was realized due to the contribution of women (p = 0.027). In the AF group, accumulation of the CT genotype was observed (49.2% versus 35.4% in the control). Men with the CT genotype and AF had higher systolic blood pressure levels and were less likely to suffer from diabetes mellitus compared to controls. The risk of developing AF in carriers of the T allele was reduced, without statistical significance, and the association changed direction to positive in carriers of the CT genotype (p = 0.063) compared with homozygous CC or TT.

Conclusion. The association between AF and CAMK2B gene at the population level has not been described in the literature, and our results indicate the potential for further research on the rs35089892 locus.

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